6-Biotin-17-NAD

6-Biotin-17-NAD (6-biotin-17-nicotinamide adenine dinucleotide) is a biotinylated chemical probe analog of nicotinamide adenine dinucleotide (NAD+) in which a biotin tag is conjugated through a 17-atom linker at the C6 position of the adenine ring. The modification preserves the essential NAD+ pharmacophore (nicotinamide ribose + ADP portion) required for substrate recognition by NAD+-consuming enzymes, while appending a biotin handle that allows streptavidin-based affinity pull-down, detection, or imaging of ADP-ribosylated proteins.

6-Biotin-17-NAD is principally used as a substrate surrogate for poly(ADP-ribose) polymerases (PARPs) in biochemical assays. PARPs cleave NAD+ at the nicotinamide-ribose bond, transferring ADP-ribose onto target proteins; the biotin tag on the ADP-ribose portion then marks modified proteins for detection with streptavidin-HRP or streptavidin-coupled beads. Related clickable NAD analogs (using azide or alkyne handles rather than biotin) have been used for chemoproteomics-scale identification of PARP substrates across the ADP-ribosylome.

Potential Research Applications: PARP enzymatic activity assays — colorimetric, fluorescent, or bead-based detection of poly(ADP-ribose) chain formation on substrate proteins (e.g., PARP3 activity profiling) ADP-ribosylated protein pull-down and substrate identification via streptavidin affinity after 6-Biotin-17-NAD incubation with cell lysates or purified PARPs NAD+/SIRT6/PARP1 axis research in DNA repair pathway studies Control substrate for comparing ADP-ribose writing and erasing (macrodomain/PARG erasers) in reconstituted repair systems

How Biotinylated NAD+ Is Used in the Literature

A 2018 SLAS Discovery paper describes a human PARP3 enzymatic assay built on biotinylated NAD+ as the substrate: PARP3 transfers the biotinylated ADP-ribose onto a histone acceptor and the product is read out with streptavidin, giving a screening-compatible activity assay for a PARP family member whose biology was still being mapped. The same logic underlies substrate-identification work. A 2010 Journal of the American Chemical Society study used clickable NAD analogs carrying a terminal alkyne to label PARP-1 and tankyrase-1 substrate proteins; conjugation of a biotin affinity tag allowed isolation and identification of 79 candidate PARP-1 substrates, including known targets such as histones and heterogeneous nuclear ribonucleoproteins. 6-Biotin-17-NAD achieves the affinity handle directly rather than through a click step, which simplifies the workflow at the cost of a bulkier substrate that some PARPs process less efficiently than native NAD+.

Where the Reagent Fits in PARP Pharmacology

Clinical PARP inhibitors are now understood to act partly by trapping PARP1 and PARP2 on damaged DNA rather than solely by blocking catalysis, and the trapping potency of niraparib, olaparib, and veliparib does not track their catalytic IC50 values. A biotinylated NAD+ substrate lets a laboratory separate those two questions: catalytic turnover is measured as streptavidin-detectable ADP-ribose incorporation, while trapping is measured independently by chromatin-fraction PARP retention. The NAD+ pool itself is a moving target in aging and metabolic models, since tissue NAD+ declines with age across rodents and humans; a labeled substrate supplied at a defined concentration removes that variable from an in vitro assay.

Live Market Data on Peptides.SO (listing data on file as of 13 September 2026)

Peptides.SO currently tracks 1 listing for 6-Biotin-17-NAD, from CPC Scientific at $302.50 (priced per mg on the listing). That is the highest per-listing price of any product in the platform's anti-aging category, where the median across 512 priced listings is $79.99, so it costs roughly 3.8 times the category midpoint. The premium reflects a multi-step nucleotide conjugation and chromatographic purification, and the fact that this is a detection reagent used in microgram quantities rather than a bulk compound. No supplier on the platform currently has a testing score or a certificate-of-analysis link on file; for a probe like this, request the vendor's HPLC purity and mass-spectrometry confirmation of the intact biotin-linker-NAD conjugate, since free biotin or unconjugated NAD+ in the lot will produce false negatives.

Frequently Asked Questions

Does 6-Biotin-17-NAD work for all 17 human PARPs? No single study has tested it across the family. It has been used with PARP1-family enzymes and in a PARP3 assay; mono-ADP-ribosyltransferases with more constrained active sites may accept it poorly. Validate each enzyme against native NAD+ before drawing conclusions.

Can it be used in live cells? The charged phosphate groups limit passive membrane permeability, so it is normally applied to permeabilized cells, nuclear extracts, lysates, or purified enzymes rather than intact cells.

How should it be stored? As a lyophilized powder at -20°C or below, protected from light and moisture. Aqueous stocks are best made fresh or stored in single-use aliquots at -80°C, since the nicotinamide-ribose bond is the same one PARPs cleave and is chemically labile.

Related Pages on Peptides.SO

For the unlabeled coenzyme, see the NAD+ price comparison and the NAD+ research profile. The DNA damage response and PARP inhibitor overview covers the pathway context. The reconstitution calculator handles molar stock preparation, and the CoA guide explains what to look for in a vendor purity document.

Cited Research

Ji M et al.. The Development of a Biotinylated NAD(+)-Applied Human Poly(ADP-Ribose) Polymerase 3 (PARP3) Enzymatic Assay. SLAS Discov. 2018. PubMed 29676938

Jiang H et al.. Clickable NAD analogues for labeling substrate proteins of poly(ADP-ribose) polymerases. J Am Chem Soc. 2010. PubMed 20560583

Murai J et al.. Trapping of PARP1 and PARP2 by Clinical PARP Inhibitors. Cancer Res. 2012. PubMed 23118055

Covarrubias AJ et al.. NAD(+) metabolism and its roles in cellular processes during ageing. Nat Rev Mol Cell Biol. 2021. PubMed 33353981

For laboratory research use only. Not for human or veterinary use, diagnosis, treatment, cure, or prevention of any disease. THIS PRODUCT IS NOT FOR HUMAN CONSUMPTION.